Human Primary Pancreatic Islets
Cat. No.
T0199
Unit
Per IEQ
Price
Inquiry
Specifications
| Description |
Human Primary Pancreatic Islets can be split into different culture vessels (i.e. multi-well plates) for experimental needs, but they will not proliferate in vitro. Experiments should be conducted within 7 days upon cell arrival. Upon thaw, the cells cannot be cryopreserved. These cells are an ideal model for long-term islet grafting survival studies and can provide insight into development of novel therapies for other pancreas related diseases. |
| Cat. No. | T0199 |
| Name | Human Primary Pancreatic Islets |
| Unit | Per IEQ |
| Price | Inquiry |
| Category | Primary Cells |
| Cell Type | Primary Cells |
| Organism | Human (H. sapiens) |
| Tissue | Pancreas |
| Donor Disease | Normal |
| Donor History | Normal tissue |
| Cell Morphology | Suspension |
| Growth Properties | Suspension, round |
| Propagation Method |
The media supplied during shipment is not sufficient for the cells and the customer will need to buy Cat. No. TM0199-A for IMMEDIATE Islet Recovery upon delivery and TM0199-B for culturing following recovery - Cat. No. TM0199-A for Islet Recovery as it enables the islets to round up with increased glucose responsiveness within the first 48 hours of recovery. - Cat. No. TM0199-B for Long Term Culturing as it is enhanced to reduce islet chaining and minimize islet fusion. - Cat. No. TM0199-C for Islet Cold Culture Media for transportation only. Carbon dioxide (CO2): 5%, Temperature: 37.0°C. |
| Growth Conditions |
Refer to detailed instructions in the following sections for islet handling: Unpacking and Storage Instructions, Thawing Protocol, Subculturing Protocol, Cryopreservation. The media supplied during shipment is not sufficient for islet maintenance. The following media should be purchased with islets: Cat. No. TM0199-A for islet recovery upon delivery; Cat. No. TM0199-B for islet culturing following recovery; Cat. No. TM0199-C for long term islet cold storage. |
| Subculture Protocol |
Instructions for Islet Media Change 1. Warm media to room temperature. 2. Angle culture vessel for at least 30 minutes to allow islets to congregate on one corner of the culture vessel. Test media after 30 minutes to ensure islets more than 50 microns in size are not visible. Allow for additional settling time until no islets are present in test sample. 3. Maintain flask position and aspirate 50% of the used media without disturbing the congregated islets. 4. Add the same volume of fresh TM0199-A or TM0199-B that was removed. 5. Incubate cells according to recommended conditions. Instructions for Short Term Islet Culture (37°C incubator, 5% CO2) 1. For cells in short-term culture, media should be changed every 2-3 days. 2. Assess viability and purity each time media is changed. Islets can be cultured up to 2 weeks using this method. |
| Application | Research Use Only. |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| BioSafety | II |
| Disclaimer |
1. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). 2. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 3. abm warrants that cells shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period". |
Documents
Supporting Protocol
STR
There are no STR for this product yet!
FAQs
| I want to make sure these cells express my gene of interest before I decide to buy the cell line. Can you provide a sample so this can be tested? | |
|
We do not perform extensive downstream characterization, or gene expression profiling of our cell lines. However, you can check the ‘References’ tab to see if a publication is available, or you can contact us to see if RNA or cell lysates are available for your cell line of interest. Please directly contact us for further information at quotes@abmgood.com.
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| Does abm's PriGrow series medium contain antibiotics? | |
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No, the medium does not contain antibiotics and needs to be supplemented by the end-user as desired.
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| Do I need to use abm's media and Applied Cell Extracellular Matrix (G422) to culture my cells? | |
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We strongly encourage using the recommended media and culture conditions described in the "Growth Conditions" section as they have been optimized for cell growth. Other supplier's media and coating conditions have not been tested.
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| What is the difference between Applied Cell Extracellular Matrix (G422) and Collagen Coating? | |
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The main component of our Applied Cell Extracellular Matrix (G422) is type I collagen specifically. For more information on abm's Applied Cell Extracellular Matrix please visit: http://www.abmgood.com/Applied-Cell-Extracellular-Matrix-G422.html.
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| Do I have to use T25 ECM-coated flasks for growing the cells? | |
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We strongly recommend thawing cells in the T25 flasks specified on the cell-specific product page to ensure optimal post-thaw recovery of the frozen cells before testing other culture vessels.
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| If I want to plate these cells to multi-well plates (e.g. 96 well plates) or dishes, how should I prepare the plates? | |
| What percentage of Trypsin-EDTA should be used to subculture cells? Can I use Trypsin-EDTA containing phenol red? | |
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The standard concentration used for subculturing is 0.25% Trypsin-EDTA, available at abm (Cat. No. TM050). Yes, Trypsin-EDTA containing phenol red can be used as it does not interfere with trypsinization.
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| How often do I need to change the media? | |
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Media should be changed every 2-3 days or as specified within the recommended growth conditions.
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| Why are these cells classified as biosafety level II? | |
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We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
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| How long can I store frozen vials? | |
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Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen vapor phase (or below -130°C) indefinitely without affecting viability.
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| What is the recommended storage temperature for cells? | |
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In general, if you received:
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| Can I substitute heat-inactivated FBS with FBS or vice versa? | |
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FBS and heat-inactivated FBS are different in their composition; they cannot be substituted for one another.
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| My cells are not detaching, what method do you recommend to trypsinize the cells? | |
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| How are live cells shipped? | |
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Live cells are shipped as a live culture in a T12.5 flask. Cells are seeded at an optimal confluency to avoid metabolic waste build up during transport. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
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| Why are cells not attaching and forming clumps after subculturing with trypsin? | |
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Cells form clumps and display attachment issues when the trypsinization agent is not effectively neutralized. To completely neutralize the trypsin agent, add an equal amount of complete growth media prior to centrifugation. Subtle changes in culture conditions, particularly in pH and the quality of serum used in the growth medium, may also affect the amount of clumping exhibited by the cells.
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| Why are my cells forming clumps after plating? | |
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It is important to ensure cell clumps are broken up through gentle re-suspension by pipetting up and down several times after pelleting cells by centrifugation. This will ensure a homogenous single cell suspension for even plating.
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| Do I need to add the selection drug to the complete growth medium? | |
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We recommend maintaining selection pressure using the drug concentration specified in the Growth Conditions section.
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| Where can I find the CoA for my product? | |
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Certificates of analysis can be found here (https://www.abmgood.com/CoA-Library.html).
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References
- Verma, G et al. "JNK1/2 regulates ER-mitochondrial Ca2+ cross-talk during IL-1β-mediated cell death in RINm5F and human primary β-cells" Mol Biol Cell 24(12):2058-71 (2013). DOI: 10.1091/mbc.E12-12-0885. PubMed: 23615449.
- Han, Z. et al. "Survivin silencing and TRAIL expression using oncolytic adenovirus increase anti-tumorigenic activity in gemcitabine-resistant pancreatic cancer cells" Apoptosis :1-14 (2015). PubMed: 26677013.
- Na, Y et al. "Potent antitumor effect of neurotensin receptor-targeted oncolytic adenovirus co-expressing decorin and Wnt antagonist in an orthotopic pancreatic tumor model" Journal of Controlled Release Part B:766–782 (2015). DOI: doi:10.1016/j.jconrel.2015.10.015. Application: cell-based assay.
- Lee, JM et al. "A new synthetic 2′-hydroxy-2,4,6-trimethoxy-5′,6′-naphthochalcone induces G2/M cell cycle arrest and apoptosis by disrupting the microtubular network of human colon cancer cells" Cancer Lett 354(2):348-54 (2014). DOI: 10.1016/j.canlet.2014.08.041. PubMed: 25193463.
- Na, Y et al. "Potent antitumor effect of neurotensin receptor-targeted oncolytic adenovirus co-expressing decorin and Wnt antagonist in an orthotopic pancreatic tumor model" J. Controlled Release 220:766-782 (2015). DOI: http://dx.doi.org/10.1016/j.jconrel.2015.10.015. Application: Virus effects.
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