SpeedySeq DNA Sequencing
Cat. No.
C199
Unit
1 Reaction
Price
$7.50
Specifications
| SKU | C199 |
| Name | SpeedySeq DNA Sequencing |
| Unit | 1 Reaction |
Documents
FAQs
| Do you pay the transport of the samples? | |
|
We do not cover the cost of shipping samples to our sequencing facility, and this is to be arranged by the end user. If you have any queries regarding shipment of DNA samples, please email technical@abmgood.com for a prompt response.
Please note that pricing is per sample with 1 primer. For the same sample with 2 primers, the cost will be 2 x C199.
|
| I plan to send plasmids for sequencing, what is the lowest conc. for submission? Some plasmids were just around 20ng/ul. Do I need to condense my plasmids? | |
|
The minimum requirement for submission of plasmid DNA for sequencing is 100ng/ul. For crude PCR samples, please submit at least 10-15ul per sample as starting material. If you require any further assistance or information please contact technical@abmgood.com
|
| What is the link for the software to read the results and how do I determine the “N” reads with the most probable sequence for that nucleotide? | |
|
The software (FinchTV) for reading the chromatograms can be downloaded for free from Geospiza:
http://www.geospiza.com/Products/finchtv.shtml
When the software is downloaded and installed, all you have to do is open the chromatogram.
ABI format chromatogram file (.ab1)
The bases and position numbers will be shown at the top of the chromatogram, so you can easily find where the N is located and check the peaks to see what the nucleotide should be.
|
| How do I open the files? | |
|
You may open .seq files, which contains the raw sequence data, using Notepad. For chromatograms, you will need to install FinchTV (http://www.geospiza.com/ftvdlinfo.html) to open .ab1 files, which contains the visualization of the sequencing reactions.
|
| Am I able to submit a sample that is 1500bp? | |
|
Yes, you can. For 1500bp sequences, you will need to provide 2 primers to cover the whole sequence, instead of 1. The service cost for this sample would be a quantity of 2 x C199.
|
References
There are no references for this product yet!