Anti-Clathrin (619-638) Antibody
Cat. No.
Y075069
Unit
100 μl
Price
$560.00
Specifications
| Cat. No. | Y075069 |
| Name | Anti-Clathrin (619-638) Antibody |
| Price | $560.00 |
| Unit | 100 μl |
| Category | Polyclonal Antibodies |
| Gene Name | Clathrin |
| Application | WB,IHC |
| Application Info | This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy ie a dilution range of 1: 100 to 1: 400. For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended. |
| Raised In | Rabbit |
| Clonality | Polyclonal |
| Reactivity | Rat |
| Description | Clathrin is a major protein component of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice to either the plasma membrane or the trans-Golgi network. These specialised organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Clathrin localises to the cytoplasmic face of coated pit and vesicle membranes. |
| Immunogen | A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine. |
| Concentration | 1ug/ul |
| Recommended Dilutions | This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy ie a dilution range of 1: 100 to 1: 400. For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended. |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Rat |
| Specificity | This antibody has been shown to be specific for clathrin. |
| Purity | Filter purified |
| Guarantee | abm guarantees that all our Anti-Clathrin (619-638) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Depositor | Biosensis |
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